Breakthrough Stem Cell Therapy with Nanoelectrodes

Introduction

In the rapidly advancing field of regenerative medicine, optimizing the behavior and function of stem cells is essential for developing effective treatments for diseases, injuries, and tissue repair. Our patented technology offers a groundbreaking approach by using nanoelectrodes to apply precise electrical stimulation to stem cells, enhancing their ability to differentiate and regenerate. This cutting-edge solution opens new possibilities for more effective, targeted therapies in regenerative medicine and stem cell research.

Current Challenges in Stem Cell Therapy

Stem cell therapies have revolutionized medicine by offering a method to repair damaged tissues and treat a variety of diseases. However, controlling the differentiation and behavior of stem cells remains a significant challenge. Traditional methods of stimulating stem cells often lack precision and fail to produce consistent results. This limits their effectiveness, making it harder for therapies to achieve the desired outcome.

Researchers and clinicians in regenerative medicine are seeking advanced techniques that can influence stem cells more reliably and efficiently. Without this level of control, stem cell-based treatments may not reach their full potential, leaving significant opportunities for medical breakthroughs untapped.

Why Choose Nanoelectrode-Based Stimulation?

Our patented technology offers a unique approach to modulating stem cells through nanoelectrodes that deliver precise electrical stimulation. Unlike traditional methods, this system allows for fine-tuned control over stem cell behavior, promoting more accurate differentiation into the desired cell types. This technology is ideal for use in regenerative medicine, where precision is critical to success.

By applying electrical stimulation at the nano-scale, this innovation enhances the regenerative capabilities of stem cells, allowing them to more effectively repair tissues or regenerate organs. The system’s design is highly adaptable, making it suitable for a wide range of therapeutic applications, from cardiovascular disease to neurological conditions and beyond.

In addition, the use of nanoelectrodes minimizes invasive procedures, offering a less disruptive and more targeted way to influence stem cells. This leads to better patient outcomes, fewer complications, and a more streamlined path to recovery.

Key Benefits

Precision Modulation: Nanoelectrodes offer precise control over stem cell behavior, improving therapy outcomes.
Enhanced Regeneration: Promotes more effective tissue repair and regeneration through targeted electrical stimulation.
Versatile Applications: Suitable for a wide range of diseases, including cardiovascular and neurological conditions.
Minimally Invasive: Reduces the need for invasive procedures, improving patient comfort and recovery time.

Transform Stem Cell Therapy with Nanoelectrode Innovation

Licensing this nanoelectrode technology offers a powerful tool for advancing stem cell therapy and regenerative medicine. By providing precise, effective control over stem cell modulation, this innovation has the potential to transform the landscape of disease treatment and tissue repair.

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Abstract
Claims

The presently disclosed subject matter relates generally to the delivery of electrical stimuli via cell-penetrating nanoelectrodes. Such electrical stimuli leads to differentiation of cells, including but not limited to adipose derived stem cells, to neural lineage, specifically to neural cells.

What is claimed is:

1. A method of modulating an adipose-derived stem cell having a cytosol, comprising exposing said cytosol to electrical stimulation, thereby modulating the cell, wherein a nanoelectrode provides said electrical stimulation.

2. The method of claim 1, wherein said modulating comprises differentiating the cell to a neural lineage.

3. The method of claim 1, wherein said modulating comprises differentiating the cell to a neural cell.

4. The method of claim 2, wherein said differentiating to neural lineage is characterized by an increase in one or more neural stem cell markers.

5. The method of claim 4, wherein said markers include one or more of Vimentin, Nestin, and PAX6.

6. The method of claim 2, wherein said differentiating to neural lineage is characterized by a decrease in one or more adipose derived stem cells markers.

7. The method of claim 6, wherein said markers include one or more of CD9, CD10, CD13, CD29, CD44, CD49, CD54, CD55, CD59, CD73, CD90, CD105, CD106, CD146, CD166, ASMA, Collagen-1, Endomucin, and Fibronectin.

8. The method of claim 7, wherein said markers include one or more of CD29 and CD44.

9. The method of claim 1, wherein said nanoelectrode penetrates the cell and is in physical contact with the cytosol.

10. The method of claim 9, wherein said electrical stimulation is pulsed direct current between about 50 mV/cm and 500 mV/cm field strength.

11. The method of claim 10, wherein the stimulation is a monophasic 100 ms square pulse at 1 Hz frequency.

12. The method of claim 1, wherein said cell is exposed to a growth factor or chemical agent, wherein said modulating comprises differentiating the cell to a neural lineage and said differentiating occurs in a shorter time compared to differentiating in the absence of said electrical stimulation.

13. The method of claim 1, wherein said cell is not exposed to a growth factor or chemical agent during said modulation.

14. The method of claim 1, wherein said nanoelectrode is a nanofin having a width of no more than about 300 nm and a length of no more than about 10 μm.

15. The method of claim 1, wherein said nanoelectrode is a conical nanopillar having a radius no larger than about 300 nm.

16. A method for differentiating an adipose-derived stem cell to a neural cell comprising applying electrical stimulation to the cytosol of the stem cell, wherein a nanoelectrode provides said electrical stimulation to the cytosol.

17. The method of claim 16, wherein said nanoelectrode penetrates the cell and is in physical contact with the cytosol.

18. The method of claim 17, wherein said electrical stimulation is pulsed direct current between about 50 mV/cm and 500 mV/cm field strength.

19. The method of claim 16, wherein said nanoelectrode is a conical nanopillar having a radius no larger than about 300 nm.

20. The method of claim 16, wherein said nanoelectrode is a nanofin having a width of no more than about 300 nm and a length of no more than about 10 μm.

Title

Application of electrical stimulation via nanoelectrodes to modulate stem cells

Inventor(s)

Shyam Aravamudhan, Komal Garde

Assignee(s)

North Carolina A&T State University

Patent #

20190359967

Patent Date

November 28, 2019