Efficient cAMP Production for Breakthrough Research

Introduction

This patented method for cAMP (cyclic adenosine monophosphate) production is designed to support industries in need of reliable, efficient production of cAMP for applications in medical research, biotechnology, and drug development. This technology offers a streamlined approach to producing this critical second messenger, which plays a key role in regulating various cellular processes, from metabolism to immune response. Companies and research institutions focused on advancing biological and medical research will find this method indispensable for achieving high-quality results in less time.

The Challenge: Efficiently Producing cAMP for Research and Therapeutics

cAMP is an essential signaling molecule that helps regulate many biological processes, including glucose metabolism, gene expression, and hormone responses. Traditionally, producing sufficient quantities of cAMP for research or therapeutic purposes has posed significant challenges. Conventional production methods often require extensive resources, time, and materials to generate cAMP in quantities sufficient for research, testing, or commercial applications. These inefficiencies slow the pace of scientific discovery and drug development, creating a need for more efficient, scalable production processes.

Streamlined cAMP Production for Breakthrough Results

This method addresses the challenges of traditional cAMP production by providing a more efficient, cost-effective solution. By optimizing the biochemical pathways involved in cAMP synthesis, this technology enables faster production with higher yields, making it ideal for laboratories and pharmaceutical companies that require large quantities of cAMP for their research. This method supports a wide range of applications, including drug discovery, gene therapy research, and studies on cellular signaling pathways. The streamlined process ensures reliable production of high-purity cAMP, accelerating the pace of scientific discovery while minimizing waste and inefficiencies.

Key Benefits for Biotechnology and Pharmaceutical Industries

For biotechnology firms, this technology offers an opportunity to enhance research and development by providing a reliable method for cAMP production, which is crucial for studying cell signaling pathways and developing therapies. Pharmaceutical companies can integrate this method into their drug discovery processes, ensuring a consistent supply of cAMP for preclinical testing, drug efficacy studies, and therapeutic development. Research institutions will benefit from faster, more efficient production of cAMP, allowing scientists to focus on innovation without the limitations of traditional production bottlenecks.

Invest in Cutting-Edge Research and Development

Licensing this efficient cAMP production technology gives your organization access to a valuable tool that enhances both research and commercial applications. By simplifying the production process for this essential signaling molecule, your company can support advancements in drug discovery, biotechnology, and medical research, while reducing costs and improving operational efficiency. This technology is a forward-looking investment for any organization dedicated to improving healthcare outcomes, driving scientific innovation, and developing groundbreaking therapies. It’s a key asset for those seeking to lead in biotechnology and pharmaceutical advancements.

The present invention provides for novel methods for measuring the levels of cyclic adenosine monophosphate (cAMP) produced by cells. Notably, the methods provided do not require that the cell membranes be disrupted. Specifically, the present invention provides for a methods of detecting and quantifying cAMP extracellularly and for kits useful in employing these methods. Furthermore the present invention also provides for a method of isolating cAMP produced by cell culture.

What is claimed is:

1. A method of producing cyclic-adenosine monophosphate (cAMP), extracellularly, from cultured cells, said method comprising;
a) providing a culture of cells in a growth medium,
b) removing the growth medium from the cells,
c) adding a cAMP collection medium,
d) incubating said cells with said collection medium without disrupting the cells’ membranes,
e) removing the collection medium from the cells, and
f) purifying the cAMP from the collection medium.
2. The method of claim 1 wherein the cells are selected from the group consisting of mammalian, avian and insect cells.
3. The method of claim 1 further comprising concentrating the collection medium prior to purifying the cAMP.
4. The method of claim 1, wherein the cells are mammalian cells; wherein the collection medium comprises a buffered saline solution, isoproterenol and imidazolidin.
5. The method of claim, 4, further comprising determining the cAMP concentration; wherein the cells are selected from the group consisting of Madin-Darby canine kidney cells, mouse C2C12 myoblasts, and human fetal mesangial cells; wherein the collection medium comprises phosphate-buffered saline (pH 7.5); wherein the collection medium is concentrated by lyophilization prior to determining the cAMP concentration, and wherein the cAMP concentration is determined by liquid scintillation.

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Title

Method for cAMP production

Inventor(s)

Ernest B. Izevbigie

Assignee(s)

Jackson State University

Patent #

6541196

Patent Date

April 1, 2003

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