Efficient Plant Growth with Micropropagation Innovation

Introduction

As the demand for ornamental plants like Alexandrian Laurel continues to rise in landscaping and horticulture, traditional propagation methods are often time-consuming and yield inconsistent results. Our micropropagation innovation offers a scientific breakthrough, providing a method to propagate Danae racemosa (Alexandrian Laurel) efficiently and consistently. This patented technique allows for the mass production of this valuable plant, meeting commercial needs while preserving its high-quality characteristics.

The Challenges of Traditional Plant Propagation

Alexandrian Laurel (Danae racemosa) is a prized ornamental plant in landscaping and garden design, known for its elegant foliage and year-round appeal. However, its propagation through traditional methods can be slow and unreliable, limiting the ability to produce enough plants to meet market demand. This creates a bottleneck for commercial growers, landscapers, and botanical centers, who need a faster, more reliable solution to meet the increasing interest in this plant.

In addition, maintaining the quality and health of the plants during large-scale propagation can be difficult using conventional methods, especially when consistency in appearance and growth characteristics is required for high-end landscaping projects. For industries reliant on mass propagation, there is a critical need for advanced, reliable methods that ensure scalability without compromising plant quality.

Micropropagation: A Breakthrough for Scalable Plant Production

Our patented micropropagation technique offers a transformative solution for growers, landscapers, and horticulturists. By utilizing this method, Danae racemosa can be propagated efficiently in large quantities, with each plant retaining the genetic qualities and aesthetic appeal of the original stock. This process takes place in controlled laboratory conditions, ensuring a high success rate and enabling large-scale production without the variability often seen in traditional propagation techniques.

The innovation also allows for disease-free propagation, reducing the risks associated with pests and pathogens that may otherwise affect plant growth. For commercial growers, this translates into higher yields, lower costs, and greater consistency in the quality of the plants produced. The ability to produce large numbers of plants rapidly gives nurseries and botanical gardens a competitive edge, allowing them to meet demand and expand their offerings.

Key Benefits

  • Efficient and Scalable: Mass propagation of Danae racemosa with consistent quality.
  • Disease-Free Growth: Ensures healthier plants by eliminating pests and pathogens during propagation.
  • High-Quality Consistency: Retains the ornamental beauty and characteristics of the original plants.
  • Versatile Applications: Ideal for commercial growers, landscapers, and botanical centers.

Grow with Confidence Using Micropropagation Innovation

Licensing this micropropagation technology provides horticulturists and commercial growers with an advanced, reliable method to propagate Alexandrian Laurel and other valuable plants. With scalable production and improved plant health, this innovation opens new possibilities for the horticulture and landscaping industries.

Alexandrian laurel (Danae racemosa L.) is a highly demanded evergreen shrub. However, seed germination often takes up to 12 to 18 months with a germination rate of only about 20%. Also, due to its slow-growth rate, it typically takes up to six years to produce a saleable one-gallon size plant. Germinating seeds in the presence of cytokinins and/or auxins increased in vitro shoot multiplication and seedling quality enhancement. Benzyladenine was found to balance seedling development by simultaneously accelerating shoot growth and slowing down root growth, whereas thidiazuron significantly promoted shoot multiplication and proliferation by producing 5-30 shoots per seed.

What is claimed is:

1. A method to produce multiple seedlings of Alexandrian laurel from one seed, the method comprising:

(a) germinating an Alexandrian laurel seed in a germination induction medium including gibberellic acid at a concentration of about 4 ppm to about 15 ppm;
(b) culturing said germinated seed in a growth induction medium including between about 1 μM and about 20 μM thidiazuron, wherein said culturing results in two or more shoots and at least one root visible from said seed;
(c) separating said multiple-shoot crown from the root(s);
(d) culturing said separated multiple-shoot crown in a basic medium;
(e) isolating an individual shoot from said multiple shoot crown and culturing said shoot in a root growth medium including a plant growth regulator to yield a plantlet having a viable shoot and root; and
(f) acclimatizing said plantlet in soil.

2. The method of claim 1, wherein

(a) said seed is germinated for at least about four weeks
(b) said germinated seed is cultured for at least about four weeks; and
(c) said basic medium is a supplemented Woody Plant medium.
3. The method of claim 2, wherein said supplemented Woody Plant medium includes between about 1% and about 1.5% by weight sucrose.
4. The method of claim 1, wherein said plant growth regulator is between about 0.1 mg/L and about 5 mg/L IBA (indole-3-butyric acid).
5. The method of claim 1, further comprising repeating steps (d), (e) and (f).
6. The method of claim 1, wherein said step of acclimatizing said plantlet in soil includes the step of acclimatizing in soil within a greenhouse.

7. A method of propagating multiple Alexandrian laurel plantlets from a single seed, the method comprising:

(a)disinfecting an Alexandrian laurel seed;
(b) germinating said seed for at least about eight weeks in a germination induction medium including gibberellic acid at a concentration of about 4 ppm to about 15 ppm;
(c) culturing said germinated seed for at least about four weeks in a growth induction medium including between about 1 μM and about 20 μM thidiazuron, wherein said culturing results in at least one root and a multiple shoot crown comprising a first individual shoot and a second individual shoot-visible from said seed;
(d) separating said first individual shoot and said second individual shoot from the root(s);
(e) culturing said first individual shoot and said second individual shoot in a root growth medium including a plant growth regulator to yield a first plantlet and a second plantlet, each plantlet having a viable shoot and root; and
(f) acclimatizing said first and second plantlets in soil.
8. The method of claim 7 wherein said step of disinfecting said seed includes the step of employing a disinfectant including an active ingredient selected from bleach, methanol, ethanol and combinations thereof.
9. The method of claim 7 wherein said separating step further includes a preceding step of selecting a dominant shoot.
10. The method of claim 7 wherein said plant growth regulator is an auxin.

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Title

Micropropagation of alexandrian laurel (Danae racemosa L)

Inventor(s)

Guochen Yang, Zhongge Lu

Assignee(s)

North Carolina A&T State University

Patent #

9474272

Patent Date

October 25, 2016

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